investigating the effect of temperature on the rate of photosynthesis experiment

and initiate the logger. The factors we will keep the same in order to make the experiment accurate are: the length of the elodea for each temperature. This is an increase of 5.6mm3/min in a range of 15 degrees.

At the optimum temperature, the enzyme is most efficient and the rate if maximum. This is because enzymes control the dark stage of photosynthesis. This is because the distance was measured by a ruler, and is only accurate to the nearest mm. Type: By looking at the class average graph and our group graph they both follow the same trend. Save my name, email, and website in this browser for the next time I comment. This means the oxygen released is only part of the oxygen produced by the plant, as some has already been used up. I predict that few if any oxygen bubbles will be produced. 2.

6. Thus the concentration of CO2 in the 9. Mean volume of oxygen released at 35oC = 34.27307692. We will also change the water baths. ATP and reduced NADP are passed from the light-dependant to the light- independent reactions. Enzymes get denatured after the optimum temperature (40oC), as its three-dimensional shape changes and intra and intermolecular bonds are broken, so that the enzyme and substrate cannot be held in place long enough to react, and because of the shapes changing they also do not bind as specifically as before. This is an example of a common experiment used to investigate light intensity and the rate of photosynthesis. The gas should be checked to prove that it is indeed oxygen - relights a glowing splint.

Factors affecting the rate of photosynthesis: The main factors affecting the rate of photosynthesis are light intensity, temperature and carbon dioxide concentration.

The amount of water in the water At high concentrations, the rate of photosynthesis begins to level out due to factors not related to carbon dioxide concentration. When exactly 15 degrees is reached, we will remove the ice cubes. Since the value of t is bigger than the critical value -the one that corresponds to a 5% probability that chance could have produced it- in the table I can be at least 95% confident that the difference between the means is significant. Start the Logger Pro software to 1. We collected a class average and did 3 T tests to compare 2 sets of results. When the elodea is placed in a water bath of 35oC, I predict the rate of photosynthesis will increase further; this is because the enzyme rubisco is working nearer to its optimum rate. The syringe pulls the bubbles through fast; therefore we need to pull very slowly to align the bubbles correctly with the graph paper beneath. We used thermometers to measure temperature, as it is relatively accurate, to the nearest degree. The light intensity varied between 6 and 7 on the light intensity meter, even thought this is not a big difference it could still be significant enough to cause inaccuracies in the results. to the environment even the two different amounts of water have the same Using a syringe, place some elodea water in the test tube along with the elodea. I found that the rate of photosynthesis increased with temperature.

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This is because most results are close to the trend line, and both graphs follow the same pattern. In some experiments we will use a beaker, some a water tub and some a water bath. This will need to be the same for every experiment. If it is 8. This was very inaccurate as even small amounts of warm water affect the temperature by a large amount.

An assumption being made in this experiment is that the gas bubbles being counted are only oxygen, and that production of oxygen is proportional to the rate of photosynthesis. difference between the two leaves, because the two leaves from the same plant At some point, a temperature is reached that is an optimum temperature.

The temperature needs to be monitored as it can change during the course of the experiment as it can vary slightly, causing inaccuracies in the results. bath in the water bath should be kepth constant for each temperature sample.

To fit the trend line exactly, the average rate of oxygen released should be around 37mm3/min. Investigate the effects of different wavelengths of light on the rate of photosynthesis Practical activities have been safety checked but not trialled by CLEAPSS. endobj Errors that could have been made during our experiment were: * The bubbles could be pulled through too fast, therefore going into the syringe, causing inaccuracies when being pushed back out. endobj The hydrogen ions combine with a carrier molecule NADP to make reduced NADP. photosynthesis so the leaves should be same size and mass. This piece of equipment was the least accurate in our experiment.

In the actual experiment I will keep the distance between the lamp and experiment constant. Therefore I predict after the optimum temperature the rate of photosynthesis will decrease, releasing a smaller volume of oxygen. photosynthesis reaction is taken and which is.

The meter was constantly fluctuating therefore it is not completely certain how constant light intensity was in each experiment. Light is absorbed by both photosystems and excited electrons are emitted from the primary pigments of both reaction centres (P680 and P700).

Before starting my investigation on how temperature affects the rate of photosynthesis I carried out some research into photosynthesis. The sum of the figures calculated in steps 3 and 4 =, 6.

During my experiment, we did 2 repeats for each temperature. The difference between the two means (step 2) divided by the figure calculated in step 6 = 29.34384615 = 8.443919812. At temperatures above 40°C the rate slows down. Therefore in our actual experiment we will do at least three repeats. Retrieved from, Type: At higher concentrations of carbon dioxide, other factors are rate limiting, such as light intensity or temperature. At low temperatures the enzymes responsibility for photosynthesis have very little energy so the rate of photosynthesis is very slow. Its temperature will be more than Objective. endobj Also as it is thin, it is hard to measure with the human eye exactly the temperature desired, which can cause inaccuracies. The time taken for the correct temperature to be reached could affect the results and cause inaccuracies, which could make the volume of oxygen released more or less than it otherwise would be. There are many possible reasons that could result in these anomalous results. The logger is then reconnected to the personal computer and the software is used again to readout the data and see the measurements as a graph, showing the profile over time, this will allow us to see any fluctuations that may occur. Check stopwatch regularly so that time does not overrun. 1 0 obj To increase the temperature until wanted temperature is gained. This because of that; two different amounts of water can give different energies

results. This is because as the temperature increases the kinetic energy the enzyme rubisco has will also increase. Temperature is measured in my experiment. To attach to the syringe and scale, in order for bubbles to be pulled through. accurate results, by using Microsoft Office Excel, the average values of the trials Copyright 2020 Leaf Group Ltd. / Leaf Group Media, All Rights Reserved. After

Therefore in our actual experiment we will do at least ten different temperatures, in order to make the results more reliable.

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